Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Background: As the state of Punjab has become endemic for dengue, this study was planned to determine susceptibility status two different classes adulticides and VGSC gene polymorphism in domain II Aedes aegypti. Methodology: Adult bioassays were performed with pyrethrum, deltamethrin malathion as per WHO protocol. AS-PCR sequencing carried out detect V1016G Kdr (Knockdown resistance) mutations. Results:Ae. aegypti from districts Ludhiana Patiala found be resistant pyrethrum. The V/ V genotype frequency higher all districts. Three known polymorphisms not recorded but one synonymous non-synonymous mutation found. Group B intron gene. For malathion, 100% four Conclusion: Thus, present findings indicate towards whereas, moderate resistance pyrethrum recorded. Therefore, more studies should various domains